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. 2011 Oct 26;2011:753917. doi: 10.1155/2011/753917

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Copyright © 2011 Jia Fei et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Downregulation of PPARs by siRNA alters ω-6 lipid-mediated induction of Egr-1 mRNA: RASMCs were transfected with SMART-pool siRNA duplexes of PPARα, PPARγ or nontargeting control siRNA, followed by treatment with ω-6 lipids for 4 and 12 hrs. Preliminary findings indicated Egr-1 mRNA levels were upregulated when PPARα was knocked down compared to PPARγ knockdown as determined by qRT-PCR. The fold change was calculated by comparing lipid treatments with vehicle controls in each siRNA group. All values were normalized to β-actin (house-keeping gene). mRNA levels were expressed as fold change ± SEM (Standard error of ΔCT mean).