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. 2011 Mar 29;22(10):1281–1291. doi: 10.1089/hum.2010.196

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Copyright 2011, Mary Ann Liebert, Inc.

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FIG. 2. — Lentiviral transduction of DC-SIGN–expressing 293T cells. (A) The 293T.DCSIGN cell line, which stably expresses human DC-SIGN, was stained with anti-DC-SIGN- phycoerythrin (PE) (1:5). Flow cytometry revealed significant DC-SIGN expression on 293T.DCSIGN (no-fill) but not parental 293T (filled). (B) Fresh viral supernatants of FUGW/AURAG, FUGW/SING, and FUGW/VSVG were normalized by p24 (70 ng) and used to transduce 2 × 10⁴ 293T.DC-SIGN (open bars) or parental 293T cells (solid bars) lacking the expression of DC-SIGN. Three days later, the transduction efficiency was measured by analyzing GFP expression using flow cytometry. Values are given as the mean of triplicates ± SE.