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. 2010 Dec 23;22(10):1225–1237. doi: 10.1089/hum.2010.012

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Copyright 2011, Mary Ann Liebert, Inc.

PMC Copyright notice

FIG. 7. — AAV2/5CMV-R88-A3GP129A–transduced CD4⁺ C8166 T cells, human PBMCs (hPBMCs), and macrophages were significantly resistant to HIV-1 replication. (A) C8166 cells, (B) phytohemaglutinin (PHA)-stimulated human PBMCs, and (C) macrophage colony-stimulating factor (M-CSF)-stimulated human macrophages (donor 1 and 2) were transduced with AAV2/5CMV-lacZ or AAV2/5CMV-R88-A3GP129A vectors for 72 hr and infected with pNL4.3-GFP⁺, nevirapine-resistant HIV-1, or the pNL4.3-Bal virus strain. At different time points after infection, the supernatants from the infected cell cultures were collected, and viral replication was monitored by measuring the level of the HIV-1 Gag-p24 antigen. The result shown is representative of two independent experiments. Means with standard errors were calculated from the results of duplicated samples from one experiment.