FIG. 2.
GUCY2D transcript levels in Gucy2e–/– eyes treated with high-titer vector. Real-time RT-PCR analyses comparing levels of GUCY2D transcript in high-titer vector-treated Gucy2e–/– eyes with endogenous Gucy2e transcript in C57BL/6J wild-type eyes and untreated Gucy2e–/– eyes. The levels of introduced GUCY2D transcript in treated eyes are 30-fold higher than those seen in C57BL/6J wild-type control eyes (p<0.01, one-way ANOVA). Transcript levels detected in untreated controls are significantly lower than the levels of GUCY2D transcript detected in treated Gucy2e–/– eyes (p<0.001, one-way ANOVA).