FIG. 3.

Cone α-transducin levels and localization are restored in Gucy2e^–/– treated eyes. C57BL/6J wild-type, Gucy2e^–/– eyes treated with low-titer (LT) vector and untreated (UT) retinas were stained with antibodies against cone α-transducin (a, red) and cone arrestin (b, green). (a) Restored cone α-transducin localization to the outer segments of cones in the treated eyes, comparable to wild-type eyes. The images in the first three columns were taken using the same microscope settings, whereas the images in the last column, designated as Gucy2e^–/– UT*, are identical to those in the Gucy2e^–/– UT column except that they were taken with a long exposure and lighting levels were manipulated in Adobe Photoshop 7.0 Elements to reveal the signal in the red channel. The results indicate substantially reduced levels of cone α-transducin in untreated Gucy2e^–/– eyes, and restored levels in treated eyes. (b) The two left-hand columns are images of retinal sections of eyes harvested and processed under normal light conditions and the right-hand columns are images of those harvested and processed in the dark. In the light, cone arrestin is localized in the cone outer segments and synaptic regions of wild-type as well as treated and untreated Gucy2e^–/– eyes. In the dark, cone arrestin is localized throughout the cone cells in wild-type eyes, and in treated and untreated Gucy2e^–/– eyes. DAPI nuclear counterstain is shown in blue. Scale bars: 15 μm. Color images available online at www.liebertonline.com/hum