Figure 1.

Alignment and minimum evolution phylogeny of Repbase Can-SINE voucher sequences. All Can-SINE vouchers begin with the designation “SINEC,” followed by species of first discovery where “Fc” = Felis catus, “CF” = Canis familiaris, “Ame” = Ailuropoda melanoleuca, and “Pv” = Phoca vitulina. (A) The initial global alignment of lysine-tRNA derived segments was estimated using the Geneious alignment module (Drummond et al. 2010) and refined by hand. Lines indicate RNA polymerase III promoter boxes A and B. (B) The neighbor-joining clustering algorithm was used to estimate phylogeny with the Tamura–Nei distance model, and branch support approximated using 1000 bootstrap replications. Feliform SINEs form a distinct clade.