Figure 3.

Tau phosphorylation was augmented in aged P301S transgenic mice. A–C) Western blots of AT8, CP13, and PHF1 in the cortex (A), hippocampus (B), and spinal cord (C) of P301S (Tg) mice at 2, 7, and 10 mo of age. D–L) Quantification of AT8 (D–F), CP13 (G–I), and PHF1 (J–L) levels in the cortex (D, G, J), hippocampus (E, H, K), and spinal cord (F, I, L), using ratio of each antibody to β-actin. P301S mice (10 mo old) had increased AT8, CP13, and PHF1 levels compared to 2- and 7-mo-old P301S mice. Data are expressed as means ± se.