Figure 6.

Oxidative stress was enhanced in the brain of P301S transgenic mice. A) Levels of MDA at 2, 7, and 10 mo. No significant difference was found between P301S (Tg) mice and their wild-type (Wt) littermates. B–D) Western blots of carbonyls (B, C) and quantification of carbonyl level (D, E) at 7 mo (B, D) and 10 mo (C, E). Levels of carbonyls in 7- and 10-mo-old P301S mice were increased in the cortex and compared to nontransgenic mice. F, G) ROS generation with FADH-linked (F) and NADH-linked substrates (G) at 7 mo. No differences were found in ROS generation. St4, state 4; St3, state 3; Rot, rotenone; Ant, antimycin. Data are expressed as means ± se.