Figure 6.
Airway epithelial chloride secretion. A) Short-circuit current in CF HBE cells. Left and center panels: Eact and UTP (100 μM) were added in control (left) and IL-4 (10 ng/ml, 24 h, center) treated CF HBE cells. Right panels: summary of Eact-induced, T16Ainh-A01-sensitive peak current (mean±se, n=6–8). *P < 0.05. ENaC was inhibited by 10 μM amiloride. B) Eact (10 μM) and UTP (100 μM) induced CaCC Cl− current measured in primary cultures of non-CF human tracheal gland (HTG) serous cells. Left and center panels: TMEM16A, CFTR, and ENaC were inhibited by pretreatment with T16Ainh-A01, CFTRinh-172, and amiloride, respectively. Inset: TMEM16A immunoblot in whole cell homogenates of CF HBE and HTG cells. Right panel: summary of UTP and Eact-induced peak current in the presence and absence of T16Ainh-A01 (mean±se, n=3). *P < 0.05.