PLS-DA score plots obtained from (A) RPLC, (B) HILIC, and (C) a combined dataset. D, Validation plot obtained from 100 permutation tests. E, T-predicted scatter plot obtained from the combined PLS-DA model (● BC, ▴ healthy controls, [daif2] BC patient prediction set, and ■ control prediction set). In total, 24 BC patients and 29 controls participated in the PLS-DA modeling process. Urine samples from three BC patients (two LG and one HG) and three healthy controls were used to test the predictive accuracy of the combined model.