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. Author manuscript; available in PMC: 2012 Dec 15.
Published in final edited form as: Int J Cancer. 2011 May 5;129(12):2807–2815. doi: 10.1002/ijc.25963

Figure 2.

Figure 2

Immunoblotting VEGFR-2 in melanoma cell extracts. a), Triplicate samples of 20 ug protein of VMM18 (VEGFR-2+) and DM122 (VEGFR-2neg) melanoma cell line extracts were resolved on the same gel and transferred to Immobilon. Separate sections of the filter were immunoblotted with antibodies A-3, 55B11, or Beta Actin as loading control. The positions of Precision Plus Kaleidoscope standards are shown by the arrows. Top to bottom, they are 250, 150, 100, 75, 50, 37, 25, 20, 15, and 10 kDa. b) VMM18 cells were transfected with control siRNA or siRNA to VEGFR-2 and aliquots of 20 ug protein were resolved on the same gel and analyzed by immunoblotting with antibodies A-3, 55B11, and Beta Actin as loading control. c) DM122 cells were mock transfected or transfected with a plasmid encoding VEGFR-2 and aliquots of 20 ug protein were resolved and analyzed by immunoblotting with antibodies A-3 and 55B11.