. Author manuscript; available in PMC: 2011 Nov 1.

Published in final edited form as: J Chromatogr A. 2007 Aug 30;1168(1-2):3–2. doi: 10.1016/j.chroma.2007.08.054

Table 10.

Qualitative comparison of different elution modes in RPLC for use as the second dimension in 2DLC

	Isocratic	Advancing Isocratic	Gradient
Analyte Re-Focusing?	No	Very little	Yes
Sensitivity of Peak Width to Pre-Column Extra-Column Volume	High	High	Low
Column Re-Equilibration Requirement?	No	No	Yes
Time from Injection to Injection	Shortest	Good	Acceptable
Peak Capacity Dependence on Retention Range	proportional to ln(Δt_R)	essentially proportional to ln(Δt_R)	proportional to Δt_R
Peak Capacity Productivity	Best at very short times	Best at very short times	Best at moderate to long times
Simplicity of Instrumentation	Least complex	More complex	Most complex
Retention Repeatability	Probably superior to gradient	Probably superior to gradient.	Good
Range in S and ln k’_w Allowed in Samples from First Dimension	Small	Larger	Very Large
Flexibility with Respect to Method Development	Least flexible	More flexible	Most flexible
Representative References	[137]	[137,139]	[10]