Table 4.

Theoretical Calculation of Optimized Peak Capacities^a Obtained with Different Column Lengths. From ref. [37].

Lb	tGc	ΔPd	nc**e	nc**/(L0.5)f
3	12	4.3	111	64
5	20	7.0	160	72
7.5	30	10.4	211	77
10	40	13.8	253	80
15	60	20.7	324	84
20	80	27.5	383	86
25	100	34.4	435	87

^aPeak capacity based on the eleven peptides on a 2.1 mm i.d. 3.5 μm Zorbax SB-C18 column. LSST parameters of the peptides and van Deemter equation parameters were obtained on a 5.0 cm × 2.1 mm 3.5 mm Zorbax SB-C18 column. Conditions: 0.40 mL/min, 40 °C, gradient from 5% ACN to 45% ACN, 0.1% TFA present in the mobile phase (v/v), 0.30 mL dwell volume, HP 1090

^bColumn length in cm

^cGradient time in min

^dPressure drop in MPa

^ePeak capacity calculated using Eq. 9

^fRatio of peak capacity relative to the square root of the column length