Table 8.
Comparison of mode combinations in 2D-LC × LC
| Mode combination | Application | Advantage | Disadvantage |
|---|---|---|---|
| IEC and RP | proteomics, peptidomics, | orthogonality | low peak capacity |
| SEC and RP | polymers, proteomics | orthogonality | low peak capacity |
| NP and RP | polymers, pharmaceuticals, oils, | orthogonality | solvent incompatibility, limited application |
| RP and RP | peptidomics, metabolomics, pharmaceuticals, foods, cosmetics | miscible solvents, broadest application, fast speed, gradient elution on both dimensions, highest peak capacity | orthogonality, strongly depend on the column choice or mobile phase choice |
| Affinity and RP | proteomics | orthogonality | off-line, low peak capacity |
| SEC and NP | polymers | orthogonality | low peak capacity |
| SEC and IEC | proteomics | orthogonality | low peak capacity |