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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Neurotoxicology. 2011 Aug 19;32(5):554–562. doi: 10.1016/j.neuro.2011.07.008

Fig. 3.

Fig. 3

RML scrapie-infected CAD5 cells are more resistant to Mn-induced apoptotic cell death. Uninfected and RML scrapie-infected CAD5 cells were treated with 300 μM Mn and the activity of caspase-3 was determined by caspase-3 specific fluorogenic substrate over time. DNA fragmentation was assayed after 24 h of Mn treatment by ELISA. A) Uninfected CAD5 cells showed a time-dependent increase in caspase-3 activity in response to Mn treatment (p<0.001), while RML scrapie-infected CAD5 cells showed no increase at 24 h. Data shown represents three repeats with a total n=7. B) DNA fragmentation was significantly increased (p<0.001) in uninfected cells treated with Mn when compared to RML scrapie-infected cells.