Fig. 4.

RML scrapie infection attenuates Mn-induced ROS generation. Uninfected and RML scrapie-infected CAD5 cells were treated with 300 μM Mn for 16, 20, and 24 h. A) Generation of Mn-induced ROS was determined by the presence of lipid peroxidation product 4-HNE. Lysate from treated cells was probed after Western blot with anti-4-HNE antibody. To ensure equal protein loading, blots were co-treated with β-actin antibody. B) Uninfected CAD5 cells showed a time-dependent increase in 4-HNE levels as determined by densitometric analysis of three individual repeats. A statistically significantly difference in increases of 4-HNE levels is seen between uninfected and infected cells at 24 h (p<0.01).