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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: Nat Immunol. 2008 Dec 7;10(1):101–108. doi: 10.1038/ni.1675

Figure 6.

Figure 6

CCL2-induced monocyte migration across MDCK cells and HUVECs. Migration of human monocytes (isolated from healthy donors) across monolayers of DARC- and mock-transfected MDCK cells (a) and HUVECs (b) seeded onto Transwell inserts; after 4 h of incubation with 35 nM CCL2, transmigrated monocytes were counted in three different compartments: adherent to the bottom side of the filter (Filter), in suspension in the lower chamber (Suspension) and adherent to the bottom of the chamber (Bottom). (a) CCL2 PMFC, replacement of fluid in the upper chamber three times during incubation. Data are representative of three (a) or two (b) independent experiments (mean and s.d. of three samples).