Table 2. Mutagenesis oligonucleotides sequence.
| Designation | Use* | Sequence (5′ to 3′)** |
| PCR1-5FRT-aph | KmR cassette | gaattcgctagcGCTGGAGCTGCTTCGAAGTTC |
| PCR1-3FRT-aph | KmR cassette | ctcgagTTCCGGGGATCCGTCGACCTGCAGTTC |
| PCR2-5FliD | fliD deletion | gaattcTCACGCACACGCTGCAGG |
| PCR2-5FliD-rev | fliD deletion | gctagcACCTAATGATGAAATTGAAGCCATGC |
| PCR3-3FliD | fliD deletion | gaattcGCTATGAACAAGTCCTGATAACAGAGGT |
| PCR3-3FliD-rev | fliD deletion | ctcgagTTAACGAGAACTCCTGGAAAGATGCTTTC GGTGAAATCTGC |
| PCR4-5FlgJ | flgK deletion | gaattcGGCGAACCCAGCTATAACGTATTTGGCG |
| PCR4-3FlgJ | flgK deletion | gctagcATTAATCAAGCTGGACATGATGGTTCC |
| PCR5-5FlgL | flgK deletion | agatctGCGTTACTGAATATTCGCTAAAGGAGAAG |
| PCR5-3FlgL | flgK deletion | ctcgagCGTATGGCCAATTACCATCGTGCGTGCG |
*KmR, Kanamycin resistance gene.
**Restriction endonuclease cleavage sites are underlined. For PCR1, EcoRI, NheI and XhoI; PCR2 and PCR 4, EcoRI and NheI; PCR3, BamHI and XhoI; PCR5, BglII and XhoI.