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. 2011 Nov 1;6(11):e25570. doi: 10.1371/journal.pone.0025570

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Prakash et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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51 Va family (red), 25 Vb family (blue) translocation unit and 3 DUF3233 (green) representative sequences were used to generate a 1000 replicate bootstrap phylogenetic tree. Eukaryotic Vb sequences representing clusters 1 and 2 were not considered in the analysis. Sequences of each family are grouped into previously reported clusters (C 1 to 11 and C 1 to 4); DUF3233 sequences form a new cluster of the autotransporter family. Numbers at the node indicate neighbour-joining bootstrap percentages. Phylogenetic analyses were conducted in MEGA4.