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. 2011 Nov 1;6(11):e26641. doi: 10.1371/journal.pone.0026641

Figure 4. Glycosylation and phosphorylation of P. metricus (wild type) hexamerins.

Figure 4

Both hexamerins are glycosylated, but only Hexamerin 2 is phosphorylated. A: Both hexamerins shift in size toward lower molecular weight after deglycosylation with PNGase F. B: The same gel stained with phosphorylation specific Pro-Q diamond stain (Invitrogen). Hexamerin 2 and vitellogenin (between 150 and 250 kDa standard bands) appear to be phosphorylated in P. metricus hemolymph. The + sign marks the deglycosylation enzyme PNGaseF.