Figure 6. Median and 25–75% interquartile ranges for RNAi phenotypes.

P. metricus larvae were assigned to a control group that received dsRNA for green fluorescent protein (gfp) or to a treatment group that received dsRNA against the hexamerin 2 gene to trigger RNA interference (hexamerin2 ⁻). The wasps were monitored during post-treatment development and isolated for bioassays on the day they emerged as adults. A: The number of days from the start of treatment with dsRNA until cocoon spinning (time to cocoon). The significance indicator denotes Mann-Whitney U-test, P = 0.017, N = 11. This test statistic is significant only if multiple testing is not accounted for. B: The number of days between cocooning and adult emergence (time cocooned). C, D: Ovary development in 13 day-old adults as (C) a binary score (active or not – categories 1 and 2 of Fig. S3) and (D) scored as the 4 developmental classes of Figure S3. E: Length in mm of adult forewings from the proximal convergence of the C cell to the distal posterior corner of the 2Rs cell. F: Number of caterpillars eaten during the post-emergence isolation period. Control gfp and treatment hexamerin2 ⁻ phenotypes differed significantly for time to cocoon.