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. 2011 Nov 1;6(11):e26390. doi: 10.1371/journal.pone.0026390

Figure 4. The site of interaction between SPARC and caspase 8 is at the N-terminus of SPARC.

Figure 4

Co-localization of the N-terminus of SPARC with caspase 8 was determined by co-immunoprecipitation (IP) studies using whole protein extracts. A) Only the recombinant N-terminus containing His-tagged fusion protein from MIP/SP-N cells co-IP with caspase 8 in a reciprocal fashion. B) Mutations in the N-terminus domain of SPARC (SP-Nmut1, SP-Nmut2) abolishes this interaction with caspase 8, and only the wild-type SP-N interacted with caspase 8. As additional controls, neither the wild type nor the mutant FS domain (SP-Fmut1, SP-Fmut2) interacted with caspase 8.