Figure 2. Expression of HIV-1 Envs in effector cells and HIV-1 receptors in stable target cells.

A. The H-AD8 and H-JRFL cells were induced for 48 hours and then metabolically labeled with ³⁵S overnight in the absence of Dox. Control cells were treated similarly but 2 µg/ml Dox was present in the medium at all times. Cell lysates and supernatants were subjected to radioimmunoprecipitation and analysis on polyacrylamide gels. B. Levels of expression of CD4 and CCR5 receptors on the two target cells were analyzed by flow cytometry. Cells were stained with secondary antibody alone as a control, or with either OKT4 (anti-CD4) antibody or 2D7 (anti-CCR5) antibody followed by the secondary antibody. Color codes are identical for both target cells. MFI, mean fluorescence intensity.