Figure 2. (A) Nucleotide sequence conservation of mammalian TOPAZ1 transcripts and (B) Conservation of PAZ and CCCH domains of TOPAZ1 protein in mammalian species.

(A) The percentage nucleotide identity between sheep TOPAZ1 cDNA and that of other mammalian species is indicated on the right-hand side. These alignments were obtained using the Vista Genome browser (http://genome.lbl.gov/vista/index.shtml). (Ba) The PAZ domain surround (in red) consisted of 58 amino acids. The percentages of identity were 96%, 78%, and 71% between sheep-cattle, sheep-human and sheep-mouse, respectively. (Bb) The CCCH domain is indicated in the green box, and consisted of 20 amino acids. Amino acid identity was 90%, 85%, and 80% between sheep-cattle, sheep-human and sheep-mouse, respectively. Identical residues are noted with an asterisk.