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. 2011 Nov 1;6(11):e26950. doi: 10.1371/journal.pone.0026950

Figure 11. Immunodetection of TOPAZ1 protein in sheep fetal ovary.

Figure 11

Immunofluorescence was performed on transversal sections of sheep 60 dpc ovaries to detect TOPAZ1 (A) and MVH (B) proteins. Pre-immune serum (PI) was also tested (C). For each section, DAPI was performed to detect nuclei (D-E-F). TOPAZ1 showed similar cellular localization as MVH (A, B). The immunolabeling of TOPAZ1 and MVH was merged with DAPI (G, H). TOPAZ1 can be detected in the cytoplasm of germ cells in ovine fetal ovary. PI serum of TOPAZ1 did not reveal any staining (C). Scale bars = 200 µm in H (applies to A–G).