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. 2011 Nov 1;6(11):e27119. doi: 10.1371/journal.pone.0027119

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Taman, Ribeiro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Adult male worms were incubated with EZLink™ sulfo-NHS-LC-biotin followed by streptavidin- FITC conjugate. Examination of biotinylated animals by confocal microscopy showed that the labeling was confined to the surface (A, B). No significant penetration of streptavidin-FITC could be seen in the internal tissues or gut (C). Western blot analyses (D) were performed on aliquots of total biotinylated proteins (total), streptavidin-purified proteins (recovered) and the flow-through (unbound). Blots were probed first with streptavidin-conjugated to HRP to verify biotin labeling and recovery of biotinylated proteins. The streptavidin-purified fraction (recovered) was subsequently probed with anti-SmGBP antibody. The relevant molecular weights are indicated.