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. 2011 Nov 1;6(11):e27120. doi: 10.1371/journal.pone.0027120

Figure 2. Flow chart describing the material harvest, RNA extraction and the usage for analysis in tobacco.

Figure 2

qPCR, real-time quantitative polymerase chain reaction; SSH, suppression subtractive hybridization. ATCE (asymmetric two-celled proembryo), ATCE/zygote subtraction; ZyA, Zygote/ATCE subtraction; STCE (symmetric two-celled proembryo), STCE/zygote subtraction; ZyS, Zygote/STCE subtraction.