Figure 6. Real-time PCR analysis for transcripts isolated from the symmetric zygote division in zygotes, asymmetric and symmetric two-celled proembryos of tobacco.

The transcripts are indicated by library ID number. The cDNAs synthesized from zygotes, asymmetric and symmetric two-celled proembryos are used as templates for PCR amplification, respectively. The expression level in zygotes is set as reference with the default value 1.