Figure 9. The ΔlasB mutant bacteria are more susceptible to SP-A-mediated aggregation in vivo.

(A) In vitro aggregation of GFP-expressing wild-type P. aeruginosa PAO1 or ΔlasB bacteria co-incubated with hSP-A and observed under fluorescent microscopy. (B) In vivo aggregation of GFP-expressing wild-type P. aeruginosa PAO1 or ΔlasB (arrows) bacteria lavaged from mouse lungs 18-hr post-infection (n = 5) observed under FLUOVIEW FV300 confocal microscope.