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. 2011 Nov 1;6(11):e27063. doi: 10.1371/journal.pone.0027063

Figure 2. Expression of CD44, TGFβRII, vimentin and E-cadherin in cancer cell lines.

Figure 2

(A) Western Blot analysis of esophageal squamous carcinoma cell lines (TE-1 -7, -8, -11, and -12), esophageal adenocarcinoma cell lines (FLO-1, OE33, SK-GT-4) and head and neck cancer cell lines (JHU-012, JHU-013) show that expression of E-cadherin and TGFβRII is associated with low levels of CD44, while loss of E-cadherin and TGFβRII correlates with up-regulation of CD44 and the EMT marker vimentin. (B) Restoration of E-cadherin, Ecad, expression by retroviral transfection of TE-8 and FLO-1 cells compared to vector control (neo) demonstrates an overall decrease in CD44 expression along with a switch to increased CD44v over CD44s (CD44v: CD44variant, CD44s: CD44 standard).