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. 2011 Nov 1;6(11):e27081. doi: 10.1371/journal.pone.0027081

Figure 2. Resveratrol inhibited TNF-α induced inflammation.

Figure 2

A. 3T3/NIH cells were pretreated 1 h with varying concentrations of resveratrol (Res) before TNF-α (10 ng/ml) treatment. MMP-9 expression in the medium was examined by gelatin zymography after 24 h treatment. B. Resveratrol (50 nM) was used to treat cells 1, 0 hour before or 1, 3 hours post TNF-α (10 ng/ml), the relative mRNA levels of MMP-9, iNOS and IL-6 were detected by real-time RT-PCR analysis at 6 hours post TNF-α treatment. (n = 3 per group, *p<0.05 vs. TNF-α+/Res-; **p<0.01 vs. TNF-α+/Res-). C. Cells were pretreated by resveratrol 1 h before TNF-α, western blotting analysis was used to detect IL-1β expression after 9 h treatment.