Skip to main content
. 2011 Nov 1;6(11):e27081. doi: 10.1371/journal.pone.0027081

Figure 4. Resveratrol decreased acetylated RelA/p65. A.

Figure 4

3T3/NIH cells were treated with TNF-α (10 ng/ml) for 0, 1/2, 3, 9, 24 h, and acetyl-NF-κBp65(Lys310) (acetyl-p65) expression was detected by western blotting analysis in cell lysis. B. Cells were pretreated by resveratrol (50 nM) in the presence of TNF-α treatment for 3 h, acetyl-NF-κBp65(Lys310) expression were examined by western blotting analysis. C. Cells were transfected with Sirt1 or control siRNA for 72 h, then pretreated with resveratrol (50 nM) in the presence of TNF-α (10 ng/ml) treatment for 3 h. Western blotting analysis was used to detect acetyl-NF-κBp65(Lys310) expression.