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. Author manuscript; available in PMC: 2012 Aug 24.
Published in final edited form as: J Am Chem Soc. 2011 Aug 3;133(33):12944–12947. doi: 10.1021/ja204266a

Figure 4.

Figure 4

Living materials were encapsulated in agarose microgels. a) Cell encapsulated microgels were sequentially patterned with responsive micromolds. For microgrooves, agarose precursor containing 3T3 fibroblasts (red) was patterned as the first gel and gel precursor containing GFP-HUVECs was patterned as the second gel. For circular and square microwells, agarose prepolymer containing HepG2 cells (red) was patterned as the first gel and the second gel contained GFP-HUVECs. b) Fluorescent microscopy images for live/dead experiment. Microgels containing 3T3 fibroblasts were encapsulated within microgrooves while HepG2-laden microgels were within circular and square microwells.