Figure 4).

Effect of taurine deficiency on Ca²⁺ transient. Cells were incubated for 48 h with medium containing or lacking 5 mM beta-alanine. The cells were then loaded with the fluorescent Ca²⁺ probe, Indo-1. Each Ca²⁺ transient parameter is expressed as a percentage of control. Values shown represent mean ± SEM of four to five different cell preparations. *Significant difference from the control value, which was fixed at 100%. [Ca²⁺]_i Intracellular calcium concentration; TR₉₀ Time required to achieve 90% relaxation