Figure 2).

Apoptosis assay (CaspaTag 3/7, Chemicon International, USA). A Fluorescence flow cytometry CaspaTag 3/7 assay indicating an abundance of active caspase-3 and caspase-7 in cystathionine γ-lyase (CSE)-wild-type (WT) and CSE-knockout (KO) smooth muscle cells at 12 h hypoxia. *P<0.05. a.u. Arbitrary units. B and C Representative dot plots indicating FL1 fluorescence versus forward scatter (FSC) in the cell populations of control and hypoxic KO samples. D Representative histogram indicating FL1 fluorescence versus cell counts of control and hypoxic KO samples. H Height