Figure 3).

Intracellular reactive oxygen species (ROS) assay (CM-H2DCFDA). A Fluorescence flow cytometry CM-H2DCFDA assay indicating intracellular ROS levels in cystathionine γ-lyase (CSE)-wild-type (WT) and CSE-knockout (KO) smooth muscle cells at 12 h hypoxia. *P<0.05; **P<0.01. a.u. Arbitrary units. B and C Representative dot plots indicating FL1 fluorescence versus forward scatter (FSC) in the cell populations of hypoxic WT and KO samples. D Representative histogram indicating FL1 fluorescence versus cell counts of hypoxic WT and KO samples. H Height