Table 1.
Gene (RT-PCR) and protein (ELISA) expression in HRPE cells exposed to monocytes.
| Cell Culture | IL-8 | MCP-1 | |
|---|---|---|---|
| RT-PCRa | RPE + MONO, 4HR | 15.3 | 6.1 |
| RPE + MONO, 24HR | 86.2 | 9.5 | |
|
| |||
| ELISAb | RPE + MONO, 0HR | 0.73 ± 0.13 | 0.3 ± 0.02 |
| RPE + MONO, 1HRc | 1.23 ± 0.02 (p=0.019) | 1.26 ± 0.14 (p=0.0022) | |
| RPE + MONO, 4HRc | 6.45 ± 0.62 (p=0.00082) | 2.49 ± 0.06 (p=0.0000047) | |
| RPE + MONO, 24HRc | 99.80 ± 8.54 (p=0.00021) | 26.29 ± 0.69 (p=0.00040) | |
| RPE + IFN-γ (+) MONO, 24 HRc | 48.61 ± 3.32 (p=0.00032) | 15.80 ± 4.04 (p=0.0000029) | |
a
Fold increase is compared to RPE + MONO, 0HR
b
Values are mean concentrations (ng/mL) ± standard error
c
p-value is compared with control RPE + MONO, 0HR
The data are representative of experiments performed 3 times on each of 3 different cell lines, n=9.