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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Mitochondrion. 2011 Aug 11;11(6):929–934. doi: 10.1016/j.mito.2011.08.003

Figure 2. Functional interaction of WT and G517V pol γ with the p55 accessory subunit.

Figure 2

Primer extension (lanes 1-10) and processivity (lanes 11-20) assays were performed using WT and G517V pol γ enzymes in the presence and absence of p55 on singly primed M13 DNA as described in Methods. Reactions contained 25 fmol substrate (all lanes), 50 fmol pol γ exo− (lanes 3-6 and 13-16, WT; lanes 7-10 and 17-20, G517V), 100 fmol p55 accessory subunit (lanes 5, 6, 9, 10, 15, 16, 19 and 20). Activity was measured at 0 mM NaCl (odd numbered lanes) and 100 mM NaCl (even numbered lanes). Lanes 1, 2, 11 and 12 had no enzyme. Arrow indicates position of 35-mer primer.