Figure 3. Itraconazole inhibits EGM-2, VEGF, and bFGF stimulated HUVEC tube formation.
HUVEC stimulated with EGM-2, 10 ng/ml VEGF, or 12 ng/ml bFGF were allowed to spontaneously form tube networks after treatment with vehicle control, 0.1 µM, 0.6 µM, or 3.0 µM itraconazole. Unstimulated (basal media) HUVEC serve as negative control. Resulting networks were visualized with calcein-AM.