Skip to main content
. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Cancer Res. 2011 Sep 7;71(21):6718–6727. doi: 10.1158/0008-5472.CAN-11-1562

Fig. 2.

Fig. 2

Identification of ARID1A regulated genes. (A) Ingenuity analysis of networks contributed by 104 differentially expressed ARID1A-regulated candidate genes identified two prominent molecular hubs, CDKN1A encoding p21 (Cip1 or WAF1) and SMAD3. A color copy is shown in Supplementary Fig. S7. (B) Western blot demonstrates that ARID1A knockdown results in reduced expression of ARID1A and p21. (C) Induction of ARID1A expression leads to upregulation of p21 protein in both HEC-1-A (left panel) and OVISE (right panel) cell lines. (D) Western blot shows significant reduction in p21 levels following knockdown with shRNA targeting p21. (E) Two different p21 shRNAs significantly increased cellular proliferation in ARID1A-induced cells as compared to control (GFP) shRNA treated ARID1A-induced HEC-1-A cells (p< 0.01).