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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Cancer Res. 2011 Sep 7;71(21):6718–6727. doi: 10.1158/0008-5472.CAN-11-1562

Fig. 3.

Fig. 3

Interaction of p53 with ARID1A/BRG1 SWI/SNF chromatin remodeling complex. (A) Endogenous p53 co-immunoprecipitates with ARID1A and BRG1 in OSE4 cells. As a control, p53 was immunoprecipitated with a mouse anti-p53 antibody and was detected by a rabbit anti-p53 antibody in immunoblotting. (B) C-terminus of ARID1A interacts with p53. V5-tagged full-length (amino acid 1–2285) or fragments of ARID1A were co-stransfected into 293FT cells with FLAG-p53 construct and were applied for immunoprecipitation using FLAG-Ab beads. (C) Coomassie stain of recombinant ARID1A-V5 (amino acid 1759–2285), GST, GST-p53 wild-type (WT) and p53 R175H mutant (left); Western blot of ARID1A-V5 after pull-down with Glutathione beads (right). Arrow heads indicate the bands corresponding to respective proteins. (D) ChIP-reChIP experiments with anti-p53 and BRG1 antibodies. Mouse IgG (mIgG) and rabbit IgG (rIgG) were used as negative controls. (E) Chromatin immunoprecipitation demonstrates that both BRG1 and p53 bind to CDKN1A and SMAD3 promoters, and that knockdown of ARID1A significantly reduces the binding of BRG1 but not p53 to these two promoters. *, p<0.05; **, p<0.01; ***, p<0.001 (t test).