Skip to main content
. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Int J Cancer. 2011 Apr 25;130(5):1029–1035. doi: 10.1002/ijc.26044

Figure 2. HtrA1 degrades XIAP at protein level.

Figure 2

A–B, cleavage of XIAP by HtrA1 in ovarian cancer cells. OV202 (A) or OV167 (B) ovarian cancer cells were transfected with indicated amounts of plasmids encoding WT HtrA1cDNA. 24 h post transfection, cell lysates were collected and XIAP levels were determined by western blot analysis. C–D, expression of XIAP mRNA remains unchanged after transfection with plasmids encoding HtrA1 cDNA. OV202 (C) and OV167 (D) cells were transfected with indicated amount of HtrA1 cDNA. RNA samples were collected at 24 hours after transfection and mRNA level of XIAP was determined by real-time RT-PCR. Bars represent mean ± s.d. of three independent experiments performed in triplicate.