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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Int J Cancer. 2011 Apr 25;130(5):1029–1035. doi: 10.1002/ijc.26044

Figure 4. CDDP induces the cleavage of XIAP by HtrA1.

Figure 4

OV167 (A) or OV202 (B) cells were transiently transfected with plasmids encoding WT HtrA1cDNA, followed by the treatment of cisplatin (CDDP) (10 μM) for 24h. HtrA1 and XI0AP levels were determined by Western-blot analysis. C down-regulation of HtrA1 leads to higher level of XIAP under CDDP treatment. SKOV3 clonal cells with NT or HtrA1 shRNA (sh1 and sh2) were treated with 10 μM CDDP for 24h and cell lysates were collected followed by Western-blot analysis.