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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Int J Biochem Cell Biol. 2011 Sep 3;43(12):1792–1801. doi: 10.1016/j.biocel.2011.08.018

Fig. 1.

Fig. 1

Stalk composition of yeast mutants. (A) Schematic representation of the ribosomal stalk in the yeast mutants used in this study. P proteins on the ribosome and in the cytosol are shown in each yeast strain. (B) Ribosomes (10 pmol) and the cytosolic fraction (40 μg) isolated from the ΔP1, ΔP2 and P0ΔAB (ΔAB) mutants and the isogenic wild type were analyzed by immunoblot analysis using polyclonal antibody against P0 and monoclonal antibodies against P1β, P2α and P2β. Anti-L3 and anti-Pgk1 were used as loading controls for the ribosome and cytosol fractions, respectively. The immunoblot analysis was repeated three times using different ribosome preparations.