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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Int J Biochem Cell Biol. 2011 Sep 3;43(12):1792–1801. doi: 10.1016/j.biocel.2011.08.018

Fig. 5.

Fig. 5

Effect of P1α/P2β heterodimers on the depurination activity of Stx1A1 and Stx2A1 on yeast ribosomes by the dual-primer extension assay. The same rRNAs (1 μg) from the reactions in Fig. 4 were used in the dual primer-extension assay. Yeast ribosomes (20 pmol) from the wild type, ΔP1 and P0ΔAB mutant (ΔAB) were incubated with 0.3 pmol of activated Stx1 or Stx2 in the presence of 30 pmol of P1α/P2β at 30°C for 30 min (Ribosome:P1α/P2β ratio of 1:1.5). The level of depurination was quantified by dual primer extension analysis. The first two lanes correspond to the primer extension products with the 25S rRNA primer only (25S) and the depurination primer only (Dep).