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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Cancer Res. 2011 Sep 15;71(21):6654–6664. doi: 10.1158/0008-5472.CAN-11-1446

Fig.3. SIRT1 interacts with ERα.

Fig.3

(A) SIRT1 was immunoprecipitated (IP) from ER− (HMEC, MCF10A, MB231 and MB453) and ER+ (MCF7 and ZR75.1) cells using SIRT1 or ERα antibodies. Immunoblotting (IB) was done with SIRT1 or ERα antibodies. (B) Colocalization of SIRT1 and ERα in MCF-7 and ZR75.1 cells. Hoechst staining was used to locate the nucleus. The scale bars represent 20 µm. (C) ER− MCF10A and MB231 cells were transfected with an expression construct of ERα. IP and IB were performed 48 h post-transfection with SIRT1 and ERα antibodies. (D) MCF10A cells were transfected with an ERα expression construct, and colocalization (arrows) was monitored with SIRT1 and ERα antibodies.