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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Cancer Res. 2011 Sep 7;71(21):6643–6653. doi: 10.1158/0008-5472.CAN-11-0744

Figure 4. US28 promotes glioma angiogenesis.

Figure 4

A. NPC transduced with either LXSN-HA-US28 or Ad-US28 and control LXSN/Mock treated cells were processed for immunofluorescence. Right panels- NPCs that express US28 (green) secrete VEGF (blue), as demonstrated by co-localization of the two markers. Nuclei are stained with propidium iodide. Bar= 100μm. B. NPC, U251, U87, and a primary GBM line ( 4121) were treated with HCMV (Towne, TR, 1 MOI), transduced with Ad-US28, or treated with EGF (50ng/ml) in serum free media. Supernatants were used in an ELISA for VEGF. Samples were assayed in quadruplicate and the experiment was repeated twice. Comparisons between treated and mock within the same cell line, were analyzed using ANOVA *, p=0.02, **, p<0.002. C. NPC-derived supernatants were tested in HUVEC tube formation assays. Complete endothelial cell growth media was used as a positive control. Representative photomicrographs are shown. Each condition was assayed in six wells of a 24 well plate and the experiment was repeated twice. Bar= 100μm. D. Average numbers of branch points and endothelial cell lumens are shown from one representative experiment. Comparisons were analyzed using ANOVA. *, p<0.02 in all cases.