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WGA-agarose affinity chromatography of the Biogel P6 fractions arising from pronase-digestion of [9-3H] sialyl HCGβ. a. Biogel P6 fractionation of Pronase digested [9-3H]sialyl HCGβ. WGA-agarose chromatography of : b. Pronase fraction A; c. Pronase fraction B; d. Pronase fraction C and e. Pronase fraction D. f. Biogel P6 chromatography of [9-3H]sialylated products arising from mild alkaline borohydride treatment of HCGβ g. TLC analysis of fractions A–C from panel f.
