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. Author manuscript; available in PMC: 2012 Nov 8.
Published in final edited form as: Biochemistry. 2011 Oct 13;50(44):9475–9487. doi: 10.1021/bi200301w

Figure 6. WGA-agarose chromatography of [9-3H] Sialyl Fetuin and the Biogel P6 fractions A, B and C arising from pronase-digested [9-3H] Sialyl Fetuin: a).

Figure 6

Biogel P6 fractionation of pronase digested [9-3H]sialyl Fetuin. WGA-agarose chromatography of b) [9-H3]sialyl Fetuin, c) Pronase fraction C, d) Pronase fraction B and e) Pronase fraction A. f) Biogel P6 elution of alkaline borohydride treated [14C] sialyl Fetuin, g) Thin layer chromatography of the Biogel P6 fractions isolated from alkaline borohydride treatment of [9-3H] Fetuin show the presence of one major fraction with radioactivity. h) TLC of dominant fraction identified in panel g (fraction B from panel f) was developed by autoradiography. i) SDS-PAGE of [14C]sialyl fetuin before and after PNGase F treatment. 10 μg (14C-sialylfetuin) was applied to lanes 1,2,3,4, and 20 μg to lanes 5,6,7,8. Lanes 1, 2, 5 and 6 contained PNGase F treated fetuin and 3, 4, 7 and 8 fetuin without PNGase F treatment.