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Apotransferrin (panel a) and haptoglobin (panel b) were sialylated using the exchange reaction. These proteins were treated with PNGaseF to remove N-glycans as indicated. 10 μg of each protein was then analyzed using SDS-PAGE with Coomassie blue staining being used to visualize protein and phosphorimaging being applied to monitor 14C-NeuAc incorporation. All samples were analyzed in duplicate.
